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Biotechnology Information rna seq data
Rna Seq Data, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Related Articles

RNA Sequencing:

Article Title: Development of a 3D-3 co-culture microbead consisting of cancer-associated fibroblasts and human umbilical vein endothelial cells for the anti-tumor drug assessment of lung cancer
Article Snippet: All RNA-seq data will be made publicly available on the National Center for Biotechnology Information (NCBI) database, with BioProject ID PRJNA1182023.

Article Title: Cytohesin-4/ARF6 facilitates the progression of acute myeloid leukemia through activating PIK3R5/PI3K/AKT pathway
Article Snippet: • RNA-seq data generated in this study have been deposited at the National Center for Biotechnology Information Sequence Read Archive data and are publicly available as of the date of publication.

Article Title: Supporting Information for HKDC1, a target of TFEB, is essential to maintain both mitochondrial and lysosomal homeostasis, preventing cellular senescence
Article Snippet: RNA-seq data used in this study have been deposited to the National Center for Biotechnology Information Gene Expression Omnibus with accession number GSE226743.

Article Title: Radiotherapy enhances anticancer CD8 T cell responses by cGAMP transfer through LRRC8A/C volume-regulated anion channels.
Article Snippet: Limin Cao, Li Wang, Zhihong Li, Xia Wei, Jinqiu Ding, Chun Zhou, Xia Chen, Zhicheng Huang, Zhugui Shao, Junchen Shen, Hongfei Lou, Keqing Zhao, Yuwei Huang, Yuanqin Yang, Han Liu, Yumeng Sun, Junling Niu, Shan Jiang, Rong Lu, Longhai Tang, Xiaoming Zhang, Haibing Zhang, Yichuan Xiao, Jianfeng Chen, Shixin Ma, Chengjiang Gao, Guangxun Meng, Li Liu, Zhaozhu Qiu, Haopeng Wang, Liufu Deng, Youqiong Ye, XinMing Jia, Huabin Li*, Hui Xiao*

Article Title: Phytoene synthase gene GbDYA modulates carotenoids accumulation and confers yellow anther and pollen antioxidant activity in cotton
Article Snippet: The RNA-seq data from this study has been deposited in the National Center for Biotechnology Information Sequence Read Archive under accession number PRJNA1223487 and Science Data CRediT authorship contribution statement Zhihan Guan: Writing – original draft, Visualization, Formal analysis, Investigation.

Article Title: Anthocyanins Prevent Mastitis Exacerbation by Inhibiting PANoptosis Activation.
Article Snippet: Data Availability Statement The RNA-seq data were deposited in theNational Center for Biotechnology Information (https://www.ncbi.nlm.nih.gov/sra/), BioProject ID was PRJNA1213991.

Article Title: Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNA.
Article Snippet: RNA-seq data are available from theNational Center for Biotechnology Information Sequencing Read Archive (SRA) database, accession code PRJNA1249431.

Article Title: Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNA
Article Snippet: RNA-seq data are available from the National Center for Biotechnology Information Sequencing Read Archive (SRA) database, accession code PRJNA1249431 .

Gene Expression:

Article Title: Development of a 3D-3 co-culture microbead consisting of cancer-associated fibroblasts and human umbilical vein endothelial cells for the anti-tumor drug assessment of lung cancer
Article Snippet: All RNA-seq data will be made publicly available on the National Center for Biotechnology Information (NCBI) database, with BioProject ID PRJNA1182023.

Article Title: Cytohesin-4/ARF6 facilitates the progression of acute myeloid leukemia through activating PIK3R5/PI3K/AKT pathway
Article Snippet: • RNA-seq data generated in this study have been deposited at the National Center for Biotechnology Information Sequence Read Archive data and are publicly available as of the date of publication.

Article Title: Supporting Information for HKDC1, a target of TFEB, is essential to maintain both mitochondrial and lysosomal homeostasis, preventing cellular senescence
Article Snippet: RNA-seq data used in this study have been deposited to the National Center for Biotechnology Information Gene Expression Omnibus with accession number GSE226743.

Article Title: Radiotherapy enhances anticancer CD8 T cell responses by cGAMP transfer through LRRC8A/C volume-regulated anion channels.
Article Snippet: Limin Cao, Li Wang, Zhihong Li, Xia Wei, Jinqiu Ding, Chun Zhou, Xia Chen, Zhicheng Huang, Zhugui Shao, Junchen Shen, Hongfei Lou, Keqing Zhao, Yuwei Huang, Yuanqin Yang, Han Liu, Yumeng Sun, Junling Niu, Shan Jiang, Rong Lu, Longhai Tang, Xiaoming Zhang, Haibing Zhang, Yichuan Xiao, Jianfeng Chen, Shixin Ma, Chengjiang Gao, Guangxun Meng, Li Liu, Zhaozhu Qiu, Haopeng Wang, Liufu Deng, Youqiong Ye, XinMing Jia, Huabin Li*, Hui Xiao*

Article Title: Phytoene synthase gene GbDYA modulates carotenoids accumulation and confers yellow anther and pollen antioxidant activity in cotton
Article Snippet: The RNA-seq data from this study has been deposited in the National Center for Biotechnology Information Sequence Read Archive under accession number PRJNA1223487 and Science Data CRediT authorship contribution statement Zhihan Guan: Writing – original draft, Visualization, Formal analysis, Investigation.

Article Title: Anthocyanins Prevent Mastitis Exacerbation by Inhibiting PANoptosis Activation.
Article Snippet: Data Availability Statement The RNA-seq data were deposited in theNational Center for Biotechnology Information (https://www.ncbi.nlm.nih.gov/sra/), BioProject ID was PRJNA1213991.

Article Title: Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNA.
Article Snippet: RNA-seq data are available from theNational Center for Biotechnology Information Sequencing Read Archive (SRA) database, accession code PRJNA1249431.

Article Title: Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNA
Article Snippet: RNA-seq data are available from the National Center for Biotechnology Information Sequencing Read Archive (SRA) database, accession code PRJNA1249431 .

Sequencing:

Article Title: Development of a 3D-3 co-culture microbead consisting of cancer-associated fibroblasts and human umbilical vein endothelial cells for the anti-tumor drug assessment of lung cancer
Article Snippet: All RNA-seq data will be made publicly available on the National Center for Biotechnology Information (NCBI) database, with BioProject ID PRJNA1182023.

Article Title: Cytohesin-4/ARF6 facilitates the progression of acute myeloid leukemia through activating PIK3R5/PI3K/AKT pathway
Article Snippet: • RNA-seq data generated in this study have been deposited at the National Center for Biotechnology Information Sequence Read Archive data and are publicly available as of the date of publication.

Article Title: Supporting Information for HKDC1, a target of TFEB, is essential to maintain both mitochondrial and lysosomal homeostasis, preventing cellular senescence
Article Snippet: RNA-seq data used in this study have been deposited to the National Center for Biotechnology Information Gene Expression Omnibus with accession number GSE226743.

Article Title: Radiotherapy enhances anticancer CD8 T cell responses by cGAMP transfer through LRRC8A/C volume-regulated anion channels.
Article Snippet: Limin Cao, Li Wang, Zhihong Li, Xia Wei, Jinqiu Ding, Chun Zhou, Xia Chen, Zhicheng Huang, Zhugui Shao, Junchen Shen, Hongfei Lou, Keqing Zhao, Yuwei Huang, Yuanqin Yang, Han Liu, Yumeng Sun, Junling Niu, Shan Jiang, Rong Lu, Longhai Tang, Xiaoming Zhang, Haibing Zhang, Yichuan Xiao, Jianfeng Chen, Shixin Ma, Chengjiang Gao, Guangxun Meng, Li Liu, Zhaozhu Qiu, Haopeng Wang, Liufu Deng, Youqiong Ye, XinMing Jia, Huabin Li*, Hui Xiao*

Article Title: Phytoene synthase gene GbDYA modulates carotenoids accumulation and confers yellow anther and pollen antioxidant activity in cotton
Article Snippet: The RNA-seq data from this study has been deposited in the National Center for Biotechnology Information Sequence Read Archive under accession number PRJNA1223487 and Science Data CRediT authorship contribution statement Zhihan Guan: Writing – original draft, Visualization, Formal analysis, Investigation.

Article Title: Anthocyanins Prevent Mastitis Exacerbation by Inhibiting PANoptosis Activation.
Article Snippet: Data Availability Statement The RNA-seq data were deposited in theNational Center for Biotechnology Information (https://www.ncbi.nlm.nih.gov/sra/), BioProject ID was PRJNA1213991.

Article Title: Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNA.
Article Snippet: RNA-seq data are available from theNational Center for Biotechnology Information Sequencing Read Archive (SRA) database, accession code PRJNA1249431.

Article Title: Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNA
Article Snippet: RNA-seq data are available from the National Center for Biotechnology Information Sequencing Read Archive (SRA) database, accession code PRJNA1249431 .

Expressing:

Article Title: Development of a 3D-3 co-culture microbead consisting of cancer-associated fibroblasts and human umbilical vein endothelial cells for the anti-tumor drug assessment of lung cancer
Article Snippet: All RNA-seq data will be made publicly available on the National Center for Biotechnology Information (NCBI) database, with BioProject ID PRJNA1182023.

Article Title: Cytohesin-4/ARF6 facilitates the progression of acute myeloid leukemia through activating PIK3R5/PI3K/AKT pathway
Article Snippet: • RNA-seq data generated in this study have been deposited at the National Center for Biotechnology Information Sequence Read Archive data and are publicly available as of the date of publication.

Article Title: Supporting Information for HKDC1, a target of TFEB, is essential to maintain both mitochondrial and lysosomal homeostasis, preventing cellular senescence
Article Snippet: RNA-seq data used in this study have been deposited to the National Center for Biotechnology Information Gene Expression Omnibus with accession number GSE226743.

Article Title: Radiotherapy enhances anticancer CD8 T cell responses by cGAMP transfer through LRRC8A/C volume-regulated anion channels.
Article Snippet: Limin Cao, Li Wang, Zhihong Li, Xia Wei, Jinqiu Ding, Chun Zhou, Xia Chen, Zhicheng Huang, Zhugui Shao, Junchen Shen, Hongfei Lou, Keqing Zhao, Yuwei Huang, Yuanqin Yang, Han Liu, Yumeng Sun, Junling Niu, Shan Jiang, Rong Lu, Longhai Tang, Xiaoming Zhang, Haibing Zhang, Yichuan Xiao, Jianfeng Chen, Shixin Ma, Chengjiang Gao, Guangxun Meng, Li Liu, Zhaozhu Qiu, Haopeng Wang, Liufu Deng, Youqiong Ye, XinMing Jia, Huabin Li*, Hui Xiao*

Article Title: Phytoene synthase gene GbDYA modulates carotenoids accumulation and confers yellow anther and pollen antioxidant activity in cotton
Article Snippet: The RNA-seq data from this study has been deposited in the National Center for Biotechnology Information Sequence Read Archive under accession number PRJNA1223487 and Science Data CRediT authorship contribution statement Zhihan Guan: Writing – original draft, Visualization, Formal analysis, Investigation.

Article Title: Anthocyanins Prevent Mastitis Exacerbation by Inhibiting PANoptosis Activation.
Article Snippet: Data Availability Statement The RNA-seq data were deposited in theNational Center for Biotechnology Information (https://www.ncbi.nlm.nih.gov/sra/), BioProject ID was PRJNA1213991.

Article Title: Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNA.
Article Snippet: RNA-seq data are available from theNational Center for Biotechnology Information Sequencing Read Archive (SRA) database, accession code PRJNA1249431.

Article Title: Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNA
Article Snippet: RNA-seq data are available from the National Center for Biotechnology Information Sequencing Read Archive (SRA) database, accession code PRJNA1249431 .

Generated:

Article Title: Development of a 3D-3 co-culture microbead consisting of cancer-associated fibroblasts and human umbilical vein endothelial cells for the anti-tumor drug assessment of lung cancer
Article Snippet: All RNA-seq data will be made publicly available on the National Center for Biotechnology Information (NCBI) database, with BioProject ID PRJNA1182023.

Article Title: Cytohesin-4/ARF6 facilitates the progression of acute myeloid leukemia through activating PIK3R5/PI3K/AKT pathway
Article Snippet: • RNA-seq data generated in this study have been deposited at the National Center for Biotechnology Information Sequence Read Archive data and are publicly available as of the date of publication.

Article Title: Supporting Information for HKDC1, a target of TFEB, is essential to maintain both mitochondrial and lysosomal homeostasis, preventing cellular senescence
Article Snippet: RNA-seq data used in this study have been deposited to the National Center for Biotechnology Information Gene Expression Omnibus with accession number GSE226743.

Article Title: Radiotherapy enhances anticancer CD8 T cell responses by cGAMP transfer through LRRC8A/C volume-regulated anion channels.
Article Snippet: Limin Cao, Li Wang, Zhihong Li, Xia Wei, Jinqiu Ding, Chun Zhou, Xia Chen, Zhicheng Huang, Zhugui Shao, Junchen Shen, Hongfei Lou, Keqing Zhao, Yuwei Huang, Yuanqin Yang, Han Liu, Yumeng Sun, Junling Niu, Shan Jiang, Rong Lu, Longhai Tang, Xiaoming Zhang, Haibing Zhang, Yichuan Xiao, Jianfeng Chen, Shixin Ma, Chengjiang Gao, Guangxun Meng, Li Liu, Zhaozhu Qiu, Haopeng Wang, Liufu Deng, Youqiong Ye, XinMing Jia, Huabin Li*, Hui Xiao*

Article Title: Phytoene synthase gene GbDYA modulates carotenoids accumulation and confers yellow anther and pollen antioxidant activity in cotton
Article Snippet: The RNA-seq data from this study has been deposited in the National Center for Biotechnology Information Sequence Read Archive under accession number PRJNA1223487 and Science Data CRediT authorship contribution statement Zhihan Guan: Writing – original draft, Visualization, Formal analysis, Investigation.

Article Title: Anthocyanins Prevent Mastitis Exacerbation by Inhibiting PANoptosis Activation.
Article Snippet: Data Availability Statement The RNA-seq data were deposited in theNational Center for Biotechnology Information (https://www.ncbi.nlm.nih.gov/sra/), BioProject ID was PRJNA1213991.

Article Title: Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNA.
Article Snippet: RNA-seq data are available from theNational Center for Biotechnology Information Sequencing Read Archive (SRA) database, accession code PRJNA1249431.

Article Title: Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNA
Article Snippet: RNA-seq data are available from the National Center for Biotechnology Information Sequencing Read Archive (SRA) database, accession code PRJNA1249431 .



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Increased IFITM and decreased DHODH expression characterize the placenta in the context of HDPs (A) Volcano plot of transcriptomic changes identified by <t>RNA-seq.</t> Transcripts highlighted in red or blue were significantly altered ( q value < 0.05). (B) Differentially expressed genes were classified by functional enrichment analysis using the Reactome pathway database and Gene Ontology (GO) biological processes or cellular components. (C) Heatmap of mitochondria-related genes downregulated in the placenta in the context of HDPs. Genes with higher expression are shown in green, and those with lower expression are shown in red. Ctrl: premature delivery, n = 5; HDP, n = 5. (D) Expression of DHODH, OPA1, DNM1L, MFN1, TFAM, and IFITM1-3 in trophoblast BeWo cells treated with forskolin (FSK, 2.5 μM) and rotenone (Rote, 50 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗ p < 0.05, ∗∗ p < 0.01 vs. FSK alone (Tukey’s test). (E) Expression of IFITMs in BeWo cells treated with FSK (2.5 μM), orludodstat (Orlu, 1 nM), or brequinar (Bre, 25 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗∗ p < 0.01 vs. Ctrl; † p < 0.05, †† p < 0.01, ††† p < 0.001 vs. FSK alone (Tukey’s test).
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Increased IFITM and decreased DHODH expression characterize the placenta in the context of HDPs (A) Volcano plot of transcriptomic changes identified by <t>RNA-seq.</t> Transcripts highlighted in red or blue were significantly altered ( q value < 0.05). (B) Differentially expressed genes were classified by functional enrichment analysis using the Reactome pathway database and Gene Ontology (GO) biological processes or cellular components. (C) Heatmap of mitochondria-related genes downregulated in the placenta in the context of HDPs. Genes with higher expression are shown in green, and those with lower expression are shown in red. Ctrl: premature delivery, n = 5; HDP, n = 5. (D) Expression of DHODH, OPA1, DNM1L, MFN1, TFAM, and IFITM1-3 in trophoblast BeWo cells treated with forskolin (FSK, 2.5 μM) and rotenone (Rote, 50 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗ p < 0.05, ∗∗ p < 0.01 vs. FSK alone (Tukey’s test). (E) Expression of IFITMs in BeWo cells treated with FSK (2.5 μM), orludodstat (Orlu, 1 nM), or brequinar (Bre, 25 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗∗ p < 0.01 vs. Ctrl; † p < 0.05, †† p < 0.01, ††† p < 0.001 vs. FSK alone (Tukey’s test).
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Increased IFITM and decreased DHODH expression characterize the placenta in the context of HDPs (A) Volcano plot of transcriptomic changes identified by <t>RNA-seq.</t> Transcripts highlighted in red or blue were significantly altered ( q value < 0.05). (B) Differentially expressed genes were classified by functional enrichment analysis using the Reactome pathway database and Gene Ontology (GO) biological processes or cellular components. (C) Heatmap of mitochondria-related genes downregulated in the placenta in the context of HDPs. Genes with higher expression are shown in green, and those with lower expression are shown in red. Ctrl: premature delivery, n = 5; HDP, n = 5. (D) Expression of DHODH, OPA1, DNM1L, MFN1, TFAM, and IFITM1-3 in trophoblast BeWo cells treated with forskolin (FSK, 2.5 μM) and rotenone (Rote, 50 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗ p < 0.05, ∗∗ p < 0.01 vs. FSK alone (Tukey’s test). (E) Expression of IFITMs in BeWo cells treated with FSK (2.5 μM), orludodstat (Orlu, 1 nM), or brequinar (Bre, 25 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗∗ p < 0.01 vs. Ctrl; † p < 0.05, †† p < 0.01, ††† p < 0.001 vs. FSK alone (Tukey’s test).
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Increased IFITM and decreased DHODH expression characterize the placenta in the context of HDPs (A) Volcano plot of transcriptomic changes identified by <t>RNA-seq.</t> Transcripts highlighted in red or blue were significantly altered ( q value < 0.05). (B) Differentially expressed genes were classified by functional enrichment analysis using the Reactome pathway database and Gene Ontology (GO) biological processes or cellular components. (C) Heatmap of mitochondria-related genes downregulated in the placenta in the context of HDPs. Genes with higher expression are shown in green, and those with lower expression are shown in red. Ctrl: premature delivery, n = 5; HDP, n = 5. (D) Expression of DHODH, OPA1, DNM1L, MFN1, TFAM, and IFITM1-3 in trophoblast BeWo cells treated with forskolin (FSK, 2.5 μM) and rotenone (Rote, 50 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗ p < 0.05, ∗∗ p < 0.01 vs. FSK alone (Tukey’s test). (E) Expression of IFITMs in BeWo cells treated with FSK (2.5 μM), orludodstat (Orlu, 1 nM), or brequinar (Bre, 25 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗∗ p < 0.01 vs. Ctrl; † p < 0.05, †† p < 0.01, ††† p < 0.001 vs. FSK alone (Tukey’s test).
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Increased IFITM and decreased DHODH expression characterize the placenta in the context of HDPs (A) Volcano plot of transcriptomic changes identified by <t>RNA-seq.</t> Transcripts highlighted in red or blue were significantly altered ( q value < 0.05). (B) Differentially expressed genes were classified by functional enrichment analysis using the Reactome pathway database and Gene Ontology (GO) biological processes or cellular components. (C) Heatmap of mitochondria-related genes downregulated in the placenta in the context of HDPs. Genes with higher expression are shown in green, and those with lower expression are shown in red. Ctrl: premature delivery, n = 5; HDP, n = 5. (D) Expression of DHODH, OPA1, DNM1L, MFN1, TFAM, and IFITM1-3 in trophoblast BeWo cells treated with forskolin (FSK, 2.5 μM) and rotenone (Rote, 50 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗ p < 0.05, ∗∗ p < 0.01 vs. FSK alone (Tukey’s test). (E) Expression of IFITMs in BeWo cells treated with FSK (2.5 μM), orludodstat (Orlu, 1 nM), or brequinar (Bre, 25 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗∗ p < 0.01 vs. Ctrl; † p < 0.05, †† p < 0.01, ††† p < 0.001 vs. FSK alone (Tukey’s test).
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Image Search Results


Increased IFITM and decreased DHODH expression characterize the placenta in the context of HDPs (A) Volcano plot of transcriptomic changes identified by RNA-seq. Transcripts highlighted in red or blue were significantly altered ( q value < 0.05). (B) Differentially expressed genes were classified by functional enrichment analysis using the Reactome pathway database and Gene Ontology (GO) biological processes or cellular components. (C) Heatmap of mitochondria-related genes downregulated in the placenta in the context of HDPs. Genes with higher expression are shown in green, and those with lower expression are shown in red. Ctrl: premature delivery, n = 5; HDP, n = 5. (D) Expression of DHODH, OPA1, DNM1L, MFN1, TFAM, and IFITM1-3 in trophoblast BeWo cells treated with forskolin (FSK, 2.5 μM) and rotenone (Rote, 50 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗ p < 0.05, ∗∗ p < 0.01 vs. FSK alone (Tukey’s test). (E) Expression of IFITMs in BeWo cells treated with FSK (2.5 μM), orludodstat (Orlu, 1 nM), or brequinar (Bre, 25 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗∗ p < 0.01 vs. Ctrl; † p < 0.05, †† p < 0.01, ††† p < 0.001 vs. FSK alone (Tukey’s test).

Journal: iScience

Article Title: DHODH regulates trophoblast fusion via IFITM-reduced plasma membrane fluidity: Implications for hypertensive disorders of pregnancy

doi: 10.1016/j.isci.2026.116163

Figure Lengend Snippet: Increased IFITM and decreased DHODH expression characterize the placenta in the context of HDPs (A) Volcano plot of transcriptomic changes identified by RNA-seq. Transcripts highlighted in red or blue were significantly altered ( q value < 0.05). (B) Differentially expressed genes were classified by functional enrichment analysis using the Reactome pathway database and Gene Ontology (GO) biological processes or cellular components. (C) Heatmap of mitochondria-related genes downregulated in the placenta in the context of HDPs. Genes with higher expression are shown in green, and those with lower expression are shown in red. Ctrl: premature delivery, n = 5; HDP, n = 5. (D) Expression of DHODH, OPA1, DNM1L, MFN1, TFAM, and IFITM1-3 in trophoblast BeWo cells treated with forskolin (FSK, 2.5 μM) and rotenone (Rote, 50 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗ p < 0.05, ∗∗ p < 0.01 vs. FSK alone (Tukey’s test). (E) Expression of IFITMs in BeWo cells treated with FSK (2.5 μM), orludodstat (Orlu, 1 nM), or brequinar (Bre, 25 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗∗ p < 0.01 vs. Ctrl; † p < 0.05, †† p < 0.01, ††† p < 0.001 vs. FSK alone (Tukey’s test).

Article Snippet: • Raw RNA-seq data derived from human placental samples and trophoblast cell lines have been deposited at the DNA DataBank of Japan (DDBJ) Sequence Read Archive as DDBJ: DRA021720 and DRA021721 and are publicly available as of the date of publication.

Techniques: Expressing, RNA Sequencing, Functional Assay

DHODH regulates IFITM expression via IRF1 (A–H) BeWo cells or DHODH-KD BeWo cells were treated with FSK (2.5 μM), Orlu (1 nM), or Bre (25 nM) for 48 h. (A) Volcano plot showing transcriptomic changes identified by RNA-seq. Transcripts highlighted in red or blue were considered differentially expressed, as indicated by an expression change ≥2-fold ( p < 0.05). (B) Correlation analysis of RNA-seq data from Orlu-, Bre-treated, and DHODH-KD cells. (C) Differentially expressed genes were classified by functional enrichment analysis using the Wiki pathway database and GO biological processes or cellular components. (D) RNA-seq was used to evaluate the expression levels of genes associated with syncytialization. (E) RNA-seq was used to evaluate the expression levels of IRF family genes. (F) Immunoblotting for IRF1, total IRF3, and p -IRF3. GAPDH was used as a loading control. Representative data from three independent experiments are shown. The graph shows the total IRF3 and p -IRF3 levels normalized to GAPDH levels from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001 vs. Ctrl (Tukey’s test). Values represent the mean ± SEM. (G) ChIP assay showing IRF1 binding to upstream regulatory regions (up to 3 kbp) of the IFITM1, IFITM2, and IFITM3 loci in BeWo cells treated with FSK alone (2.5 μM) for 48 h. ∗ p < 0.05 vs. Ctrl; † p < 0.05, †† p < 0.01, ††† p < 0.001 vs. FSK alone (Tukey’s test). (H) Immunofluorescence staining of IRF1 (red). Nuclei were counterstained with DAPI (blue). Scale bars, 5 μm. The graph shows the number of staining cells from three independent experiments. Values represent the mean ± SEM. ∗∗∗ p < 0.001 vs. FSK.

Journal: iScience

Article Title: DHODH regulates trophoblast fusion via IFITM-reduced plasma membrane fluidity: Implications for hypertensive disorders of pregnancy

doi: 10.1016/j.isci.2026.116163

Figure Lengend Snippet: DHODH regulates IFITM expression via IRF1 (A–H) BeWo cells or DHODH-KD BeWo cells were treated with FSK (2.5 μM), Orlu (1 nM), or Bre (25 nM) for 48 h. (A) Volcano plot showing transcriptomic changes identified by RNA-seq. Transcripts highlighted in red or blue were considered differentially expressed, as indicated by an expression change ≥2-fold ( p < 0.05). (B) Correlation analysis of RNA-seq data from Orlu-, Bre-treated, and DHODH-KD cells. (C) Differentially expressed genes were classified by functional enrichment analysis using the Wiki pathway database and GO biological processes or cellular components. (D) RNA-seq was used to evaluate the expression levels of genes associated with syncytialization. (E) RNA-seq was used to evaluate the expression levels of IRF family genes. (F) Immunoblotting for IRF1, total IRF3, and p -IRF3. GAPDH was used as a loading control. Representative data from three independent experiments are shown. The graph shows the total IRF3 and p -IRF3 levels normalized to GAPDH levels from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001 vs. Ctrl (Tukey’s test). Values represent the mean ± SEM. (G) ChIP assay showing IRF1 binding to upstream regulatory regions (up to 3 kbp) of the IFITM1, IFITM2, and IFITM3 loci in BeWo cells treated with FSK alone (2.5 μM) for 48 h. ∗ p < 0.05 vs. Ctrl; † p < 0.05, †† p < 0.01, ††† p < 0.001 vs. FSK alone (Tukey’s test). (H) Immunofluorescence staining of IRF1 (red). Nuclei were counterstained with DAPI (blue). Scale bars, 5 μm. The graph shows the number of staining cells from three independent experiments. Values represent the mean ± SEM. ∗∗∗ p < 0.001 vs. FSK.

Article Snippet: • Raw RNA-seq data derived from human placental samples and trophoblast cell lines have been deposited at the DNA DataBank of Japan (DDBJ) Sequence Read Archive as DDBJ: DRA021720 and DRA021721 and are publicly available as of the date of publication.

Techniques: Expressing, RNA Sequencing, Functional Assay, Western Blot, Control, Binding Assay, Immunofluorescence, Staining